|
| MOQ: | we produce both liquid and lyophilized kit |
| Price: | USD |
| Standard Packaging: | Carton Package |
| Delivery Period: | depending on order quantity |
| Payment Method: | L/C, T/T |
| Supply Capacity: | 100,000 per day |
Feline Cat Helicobacter pylori (Hp) Nucleic Acid Detection Kit ( PCR-Fluorescent Probe )
Intend for Use:
This kit is suitable for the specific detection of pathogenic Helicobacter pylori (Hp) in cat/dog feces samples.
Principle:
This kit selection of helicobacter pylori (Hp) specific fragment to design primers probe probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, Fluorescence was emitted and detected by fluorescence PCR instrument to achieve the detection of Helicobacter pylori nucleic acid (Hp) in the completely closed reaction system.
Component:
| Composition | Quantity |
| PCR Liquid | 8 tube |
| Positive control | 1 tube |
| Negative control | 1 tube |
PCR amplification and fluorescence detection (PCR detection area):
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
| Step | Cycle number | Temperature | Time |
| 1 | 1 | 94℃ | 3min |
| 2 | 45 | 94℃ | 10s |
| 60℃ | 20s collection fluorescent |
FAM was selected for detection channel
Positive judgment value or reference interval:
| Results | Ct Value |
| Positive | Ct≤38 |
| Negative | No Ct value or Ct value≥45 |
| Gray area | 38＜Ct<45 |
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
Product performance index:
1. Accuracy
The positive reference materials of testing enterprises were all positive.
2. Minimum detection limit: the detection limit of this kit is 10copies/μL.
3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.
4. Linear, linear correlation coefficient | r | >0.980.
Product picture:
Details please check from IFU!
|
|
| MOQ: | we produce both liquid and lyophilized kit |
| Price: | USD |
| Standard Packaging: | Carton Package |
| Delivery Period: | depending on order quantity |
| Payment Method: | L/C, T/T |
| Supply Capacity: | 100,000 per day |
Feline Cat Helicobacter pylori (Hp) Nucleic Acid Detection Kit ( PCR-Fluorescent Probe )
Intend for Use:
This kit is suitable for the specific detection of pathogenic Helicobacter pylori (Hp) in cat/dog feces samples.
Principle:
This kit selection of helicobacter pylori (Hp) specific fragment to design primers probe probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, Fluorescence was emitted and detected by fluorescence PCR instrument to achieve the detection of Helicobacter pylori nucleic acid (Hp) in the completely closed reaction system.
Component:
| Composition | Quantity |
| PCR Liquid | 8 tube |
| Positive control | 1 tube |
| Negative control | 1 tube |
PCR amplification and fluorescence detection (PCR detection area):
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
| Step | Cycle number | Temperature | Time |
| 1 | 1 | 94℃ | 3min |
| 2 | 45 | 94℃ | 10s |
| 60℃ | 20s collection fluorescent |
FAM was selected for detection channel
Positive judgment value or reference interval:
| Results | Ct Value |
| Positive | Ct≤38 |
| Negative | No Ct value or Ct value≥45 |
| Gray area | 38＜Ct<45 |
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
Product performance index:
1. Accuracy
The positive reference materials of testing enterprises were all positive.
2. Minimum detection limit: the detection limit of this kit is 10copies/μL.
3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.
4. Linear, linear correlation coefficient | r | >0.980.
Product picture:
Details please check from IFU!
